Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit
Product Specifications
Target
ALT
Type
Fluorometric method
Field of Research
Liver and Renal Function
Assay Principle
ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.
Assay Performance Time
75 min
Sample Type
Serum; plasma; animal tissue
Detection Range
0.01-0.83 U/L
Sensitivity
0.01 U/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Citation 01
https://www.sciencedirect.com/science/article/pii/S1742706120307509
Citation 02
https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13332
Citation 03
https://pubs.acs.org/doi/abs/10.1021/acsami.9b19518
Citation 04
https://www.sciencedirect.com/science/article/pii/S0278691521001575
Citation 05
https://www.mdpi.com/2039948
Citation 06
https://www.nature.com/articles/s41598-022-21816-x
Inter CV
9.8%
Intra CV
2.3%
Detection Instrument
Fluorescence Microplate Reader
Available Sizes
Documents
Curated Selection
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