Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit
Product Specifications
Type
Fluorometric method
Field of Research
Glycolysis and Lipid Metabolism
Assay Principle
Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE) . Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.
Assay Performance Time
20 min
Sample Type
Serum; plasma; animal tissue; cells
Detection Range
0.12-30 μmol/L
Sensitivity
0.12 μmol/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Citation 01
https://www.mdpi.com/1908036
Inter CV
7.3%
Intra CV
1.7%
Detection Instrument
Fluorescence Microplate Reader
Available Sizes
Frequently Asked Questions
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