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Recombinant Helicobacter pylori Glutamate racemase (murI)

Product Specifications

Product Name Alternative

MurI; HPG27_509; Glutamate racemase; EC 5.1.1.3

Abbreviation

Recombinant Helicobacter pylori murI protein

Gene Name

MurI

UniProt

B5Z6S2

Expression Region

1-255aa

Organism

Helicobacter pylori (strain G27)

Target Sequence

MKIGVFDSGVGGFSVLKSLLKAQLFDEIIYYGDSARVPYGTKDPTTIKQFGLEALDFFKPHQIKLLIVACNTASALALEEMQKHSKIPIVGVIEPSILAIKQQVKEKNAPILVLGTKATIQSNAYDNALKQQGYLNVSHLATSLFVPLIEESILEGELLETCMRYYFTPLKILPEVIILGCTHFPLIAQKIEGYFMEHFALSTPPLLIHSGDAIVEYLQQKYALKKNAHAFPKVEFHASGDVIWLEKQAKEWLKL

Tag

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Cardiovascular

Relevance

Provides the-glutamate required for cell wall biosynthesis.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

35.9 kDa

References & Citations

"The complete genome sequence of Helicobacter pylori strain G27." Baltrus D.A., Amieva M.R., Covacci A., Lowe T.M., Merrell D.S., Ottemann K.M., Stein M., Salama N.R., Guillemin K. J. Bacteriol. 191:447-448 (2009)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length

Available Sizes

Curated Selection

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