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One-component TMB Substrate

Product Specifications

Applications

ELISA

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/article/10.3390/nano12152551

Citation 02

https://www.mdpi.com/1012212

Citation 03

https://www.mdpi.com/1675836

Citation 04

https://portlandpress.com/bioscirep/article-abstract/39/12/BSR20193094/221348

Citation 05

https://peerj.com/articles/5056

Recommended Usage

TMB is a popular chromogenic substrate for HRP detection in ELISA and is available in several formats. The soluble One-component TMB Substrate are supplied as Ready-to-Use mono-component liquids that require no preparation before use. The product mainly contains 3,3’,5,5’-tetramethylbenzidine and contains no DMF or DMSO. One-component TMB Substrate yields a blue color Amax= 370 nm and 652 nm) when reacted with peroxidase in microwell applications. The product changes to yellow Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Available Sizes

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