One-component TMB Substrate

TMB is a popular chromogenic substrate for HRP detection in ELISA and is available in several formats. The soluble One-component TMB Substrate are supplied as Ready-to-Use mono-component liquids that require no preparation before use. The product mainly contains 3,3’,5,5’-tetramethylbenzidine and contains no DMF or DMSO. One-component TMB Substrate yields a blue color Amax= 370 nm and 652 nm) when reacted with peroxidase in microwell applications. The product changes to yellow Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.