Rat PGF (Placental Growth Factor) CLIA Kit
Product Specifications
Abbreviation
PGF
UniProt
Q63434
Cross Reactivity
This kit recognizes Rat PGF in samples.No significant cross-reactivity or interference between Rat PGF and analogues was observed
Target
PLGF; PGFL; PlGF-2; SHGC-10760; D12S1900
Type
Sandwich-ELISA
Applications
CLIA
Field of Research
Cancer; Cardiovascular; Metabolism
Assay Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PGF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PGF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat PGF. You can calculate the concentration of Rat PGF in the samples by comparing the RLU value of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
3.13-200 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
1.88 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,6 months
Product Datasheet
https://789.bio/ea/dubpmd
Product MSDS
https://789.bio/eb/yz9OG4
Species
Rat
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Rat PGF concentrations in serum, plasma and other biological fluids.
Available Sizes
Curated Selection
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