Rat CREB1 (Cyclic AMP Response Element Binding Protein 1) CLIA Kit
Product Specifications
Abbreviation
CREB1
UniProt
P15337
Cross Reactivity
This kit recognizes Rat CREB1 in samples.No significant cross-reactivity or interference between Rat CREB1 and analogues was observed
Type
Sandwich-ELISA
Applications
CLIA
Field of Research
Cancer; Epigenetics and Nuclear Signaling; Immunology
Assay Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CREB1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CREB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CREB1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat CREB1. You can calculate the concentration of Rat CREB1 in the samples by comparing the RLU value of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
62.5-4000 fmol/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
37.5 fmol/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,6 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S037887412100221X
Species
Rat
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Rat CREB1 concentrations in Serum, plasma and other biological fluids.
Available Sizes
Frequently Asked Questions
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