Rat CNTF (Ciliary Neurotrophic Factor) CLIA Kit
Product Specifications
Abbreviation
CNTF
UniProt
P20294
Cross Reactivity
This kit recognizes Rat CNTF in samples.No significant cross-reactivity or interference between Rat CNTF and analogues was observed
Target
CNTF
Type
Sandwich-ELISA
Applications
CLIA
Field of Research
Cancer; Neuroscience; Stem cells
Assay Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CNTF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CNTF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CNTF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat CNTF. You can calculate the concentration of Rat CNTF in the samples by comparing the RLU value of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
12.5-800 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
7.5 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,6 months
Species
Rat
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Rat CNTF concentrations in serum, plasma and other biological fluids.
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
