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Mouse MYO (Myoglobin) CLIA Kit

Product Specifications

Abbreviation

MYO

UniProt

P04247

Cross Reactivity

This kit recognizes Mouse MYO in samples.No significant cross-reactivity or interference between Mouse MYO and analogues was observed

Target

MB; PVALB

Type

Sandwich-ELISA

Applications

CLIA

Field of Research

Cancer; Cardiovascular; Metabolism

Assay Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MYO. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MYO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MYO, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse MYO. You can calculate the concentration of Mouse MYO in the samples by comparing the RLU value of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C/-20°C,6 months

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse MYO concentrations in Serum, plasma and other biological fluids.

Available Sizes

Frequently Asked Questions

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