Human NGF (Nerve Growth Factor) CLIA Kit
Product Specifications
Abbreviation
NGF
UniProt
P01138
Cross Reactivity
This kit recognizes Human NGF in samples.No significant cross-reactivity or interference between Human NGF and analogues was observed
Target
Beta-NGF; HSAN5; NGFB; NGF-B
Type
Sandwich-ELISA
Applications
CLIA
Field of Research
Cancer; Neuroscience
Assay Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NGF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human NGF. You can calculate the concentration of Human NGF in the samples by comparing the RLU value of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.81-500 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
4.69 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,6 months
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human NGF concentrations in serum, plasma and other biological fluids.
Available Sizes
Frequently Asked Questions
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