Human ANXA5 (Annexin A5) CLIA Kit
Product Specifications
Abbreviation
ANXA5
UniProt
P08758
Cross Reactivity
This kit recognizes Human ANXA5 in samples.No significant cross-reactivity or interference between Human ANXA5 and analogues was observed
Target
Annexin V; ANX5; ENX2; PP4; RPRGL3
Type
Sandwich-ELISA
Applications
CLIA
Field of Research
Cancer; Cell Biology; Cardiovascular
Assay Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANXA5. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANXA5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANXA5, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ANXA5. You can calculate the concentration of Human ANXA5 in the samples by comparing the RLU value of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
62.5-4000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
37.5 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,6 months
Product Datasheet
https://789.bio/ea/jvjbr9
Product MSDS
https://789.bio/eb/PtDlyL
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human ANXA5 concentrations in Serum, plasma and other biological fluids.
Available Sizes
Curated Selection
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