Human C5a (Complement Component 5a) CLIA Kit
Product Specifications
Abbreviation
C5a
UniProt
P01031
Cross Reactivity
This kit recognizes Human C5a in samples.No significant cross-reactivity or interference between Human C5a and analogues was observed
Target
C5a
Type
Sandwich-ELISA
Applications
CLIA
Field of Research
Cell Biology; Metabolism; Immunology; Signal transduction
Assay Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C5a. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human C5a. You can calculate the concentration of Human C5a in the samples by comparing the RLU value of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.63-1000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
9.38 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,6 months
Product Datasheet
https://789.bio/ea/kkulg4
Product MSDS
https://789.bio/eb/rf5KKC
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human C5a concentrations in serum, plasma and other biological fluids.
Available Sizes
Curated Selection
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