QuicKey Pro Rat Pg (Progesterone) ELISA Kit

Product Specifications

Abbreviation

Cross Reactivity

This kit recognizes Rat Pg in samples.No significant cross-reactivity or interference between Rat Pg and analogues was observed

Target

Progesterone; Pg

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal Transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat Pg are added to the micro ELISA plate wells. Rat Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat Pg in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

7.01 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0144861722002089

Citation 02

https://www.sciencedirect.com/science/article/pii/S026974912300177X

Citation 03

https://www.mdpi.com/2076-3921/11/10/1879

Citation 04

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8439717/

Citation 05

https://europepmc.org/article/pmc/pmc9520912?javascript_support=no

Citation 06

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo01744f

Citation 07

https://www.sciencedirect.com/science/article/pii/S0009279723000662

Citation 08

https://www.sciencedirect.com/science/article/pii/S0300483X22002633

Citation 09

https://www.sciencedirect.com/science/article/pii/S0147651320306047

Citation 10

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734354/

Citation 11

https://www.mdpi.com/1134966

Citation 12

https://academic.oup.com/biolreprod/advance-article-abstract/doi/10.1093/biolre/ioac192/6794001

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat Pg concentrations in serum, plasma.

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

241609-01 100 mg

(R) -Boc-2-amino-3-propargylsulfanyl-propionic acid·DCHA 98+% (HPLC)

Ask

View Details

241609-02 250 mg

(R) -Boc-2-amino-3-propargylsulfanyl-propionic acid·DCHA 98+% (HPLC)

Ask

View Details

241609-03 1 g

(R) -Boc-2-amino-3-propargylsulfanyl-propionic acid·DCHA 98+% (HPLC)

Ask

View Details

TS404523P5 25 Slides

WFDC5 CytoSection

Ask

View Details

MBS7235545-01 48 Well

Mouse ARF GTPase-activating protein GIT2 (GIT2) ELISA Kit

Ask

View Details

MBS7235545-02 96 Well

Mouse ARF GTPase-activating protein GIT2 (GIT2) ELISA Kit

Ask

View Details