QuicKey Pro Rat E3 (Estriol) ELISA Kit

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat E3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat E3 are added to the micro ELISA plate wells. Rat E3 in samples (or standards) competes with a fixed amount of E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to E3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat E3 in the samples is then determined by comparing the OD of the samples to the standard curve.