QuicKey Pro Rat CORT (Corticosterone) ELISA Kit

Product Specifications

Abbreviation

Cort

Cross Reactivity

This kit recognizes Rat Cort in samples.No significant cross-reactivity or interference between Rat Cort and analogues was observed

Target

Corticosterone; CORT

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cell biology

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Cort. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat Cort are added to the micro ELISA plate wells. Rat Cort in samples (or standards) competes with a fixed amount of Cort on the solid phase supporter for sites on the HRP linked detection antibody specific to Cort. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat Cort in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, Plasma, Urine, Saliva

Detection Range

4.69-300 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

1.87 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S221112472200211X

Citation 02

https://www.sciencedirect.com/science/article/pii/S0269749122020589

Citation 03

https://www.sciencedirect.com/science/article/pii/S004896972100262X

Citation 04

https://europepmc.org/article/pmc/pmc9520912?javascript_support=no

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6782009/

Citation 06

https://link.springer.com/article/10.1007/s12035-015-9679-z

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8635751/

Citation 08

https://link.springer.com/article/10.1007/s10753-021-01545-5

Citation 09

https://www.sciencedirect.com/science/article/pii/S0378874122003543

Citation 10

https://www.sciencedirect.com/science/article/pii/S2212429221002996

Citation 11

https://academic.oup.com/endo/advance-article-abstract/doi/10.1210/endocr/bqac042/6580263

Citation 12

https://www.sciencedirect.com/science/article/pii/S0753332220311860

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat Cort concentrations in serum, plasma, saliva, urine.

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