QuicKey Pro Mouse E2 (Estradiol) ELISA Kit

Product Specifications

Abbreviation

Cross Reactivity

This kit recognizes Mouse E2 in samples.No significant cross-reactivity or interference between Mouse E2 and analogues was observed

Target

Oestradiol; E2;17β-Estradiol; Estra-1;3;5 (10) -triene-3;17β-diol

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signaling transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse E2 are added to the micro ELISA plate wells. Mouse E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, Plasma, Urine, Saliva

Detection Range

3.13-200 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

1.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0304389421017829

Citation 02

https://www.sciencedirect.com/science/article/pii/S0269749122016499

Citation 03

https://www.nature.com/articles/s41419-021-04277-4

Citation 04

https://www.sciencedirect.com/science/article/pii/S0269749121011398

Citation 05

https://www.sciencedirect.com/science/article/pii/S0160412020322534

Citation 06

https://www.sciencedirect.com/science/article/pii/S0147651322007679

Citation 07

https://www.tandfonline.com/doi/abs/10.1080/17435390.2018.1537411

Citation 08

https://www.sciencedirect.com/science/article/pii/S0269749119332531

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc7993734/

Citation 10

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo04239d

Citation 11

https://www.nature.com/articles/srep21776/

Citation 12

https://rbej.biomedcentral.com/articles/10.1186/s12958-021-00702-7

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse E2 concentrations in Serum, Plasma, Urine, Saliva.

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