QuicKey Pro Mouse IgG (Immunoglobulin G) ELISA Kit

Product Specifications

Abbreviation

IgG

Cross Reactivity

This kit recognizes Mouse IgG in samples.No significant cross-reactivity or interference between Mouse IgG and analogues was observed

Target

Immunoglobulin G; IgG

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG are added to the micro ELISA plate wells. Mouse IgG in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IgG.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse IgG. The concentration of Mouse IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.51 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8812323/

Citation 02

https://link.springer.com/article/10.1186/s12979-018-0142-7

Citation 03

https://www.nature.com/articles/s42003-023-04454-5

Citation 04

https://www.sciencedirect.com/science/article/pii/S2589004222006757

Citation 05

https://www.nature.com/articles/srep34079

Citation 06

https://academic.oup.com/jn/advance-article-abstract/doi/10.1093/jn/nxab328/6391405

Citation 07

https://www.sciencedirect.com/science/article/pii/S1818087617309704

Citation 08

https://pubs.rsc.org/en/content/articlehtml/2020/fo/d0fo01841d

Citation 09

https://www.sciencedirect.com/science/article/pii/S1756464622001402

Citation 10

https://www.sciencedirect.com/science/article/pii/S1756464622000901

Citation 11

https://www.mdpi.com/1603134

Citation 12

https://www.mdpi.com/2072-6651/12/12/794

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse IgG concentrations in serum, plasma.

Available Sizes

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