QuicKey Pro Mouse T (Testosterone) ELISA Kit

Product Specifications

Abbreviation

Cross Reactivity

This kit recognizes Mouse T in samples.No significant cross-reactivity or interference between Mouse T and analogues was observed

Target

Testosterone; T

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal Transduction; Signal Transduction; Epigenetics and Nuclear Signaling; Developmental Biology

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse T are added to the micro ELISA plate wells. Mouse T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse T in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.14 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0304389421017829

Citation 02

https://www.sciencedirect.com/science/article/pii/S0304389420314199

Citation 03

https://link.springer.com/article/10.1186/s12989-022-00453-2

Citation 04

https://www.sciencedirect.com/science/article/pii/S0304389420323062

Citation 05

https://www.sciencedirect.com/science/article/pii/S0147651323001999

Citation 06

https://www.sciencedirect.com/science/article/pii/S0045653520323730

Citation 07

https://www.sciencedirect.com/science/article/pii/S0269749120363156

Citation 08

https://www.sciencedirect.com/science/article/pii/S0048969720345782

Citation 09

https://www.sciencedirect.com/science/article/pii/S0753332222004504

Citation 10

https://www.dovepress.com/pseudoephedrine-nanoparticles-alleviate-adriamycin-induced-reproductiv-peer-reviewed-fulltext-article-IJN

Citation 11

https://europepmc.org/article/pmc/pmc9691951?javascript_support=no

Citation 12

https://www.sciencedirect.com/science/article/pii/S0278691523000364

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse T concentrations in serum, plasma.

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