QuicKey Pro Mouse CORT (Corticosterone) ELISA Kit

Product Specifications

Abbreviation

Cort

Cross Reactivity

This kit recognizes Mouse Cort in samples.No significant cross-reactivity or interference between Mouse Cort and analogues was observed

Target

Corticosterone; CORT

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cell biology

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse Cort. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse Cort are added to the micro ELISA plate wells. Mouse Cort in samples (or standards) competes with a fixed amount of Cort on the solid phase supporter for sites on the HRP linked detection antibody specific to Cort. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse Cort in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, Plasma, Urine, Saliva

Detection Range

12.5-800 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.98 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0092867422015379

Citation 02

https://www.sciencedirect.com/science/article/pii/S2352289522000674

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1002/cjoc.202000652

Citation 04

https://www.hindawi.com/journals/omcl/2022/6877283/

Citation 05

https://link.springer.com/article/10.1186/s13020-021-00492-5

Citation 06

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d2fo01164f

Citation 07

https://pubs.rsc.org/en/content/articlelanding/2021/fo/d1fo01608c

Citation 08

https://www.sciencedirect.com/science/article/pii/S0009279722002447

Citation 09

https://link.springer.com/content/pdf/10.1007/s12035-014-9007-z.pdf

Citation 10

https://www.sciencedirect.com/science/article/pii/S0753332220307964

Citation 11

https://www.sciencedirect.com/science/article/pii/S0022395621006142

Citation 12

https://www.sciencedirect.com/science/article/pii/S0306453020305060

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse Cort concentrations in serum, plasma, saliva, urine.

Available Sizes

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