QuicKey Pro Human IGF-1 (Insulin-like Growth Factor 1) ELISA Kit

Product Specifications

Abbreviation

IGF-1

UniProt

P05019

Cross Reactivity

This kit recognizes Human IGF-1 in samples.No significant cross-reactivity or interference between Human IGF-1 and analogues was observed

Target

Insulin-like growth factor 1; IGF1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Developmental biology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF-1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IGF-1 are added to the micro ELISA plate wells. Human IGF-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IGF-1.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human IGF-1. The concentration of Human IGF-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

3.12-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

1.01 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0304383521003323

Citation 02

https://www.hindawi.com/journals/sci/2021/5553676/

Citation 03

https://www.nature.com/articles/s41419-019-1670-6

Citation 04

https://www.nature.com/articles/s41598-021-87583-3

Citation 05

https://link.springer.com/article/10.1007/s10803-020-04820-w

Citation 06

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc7502406/

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467354/

Citation 08

https://jme.bioscientifica.com/view/journals/jme/aop/jme-22-0193/jme-22-0193.xml

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218643/

Citation 10

https://www.sciencedirect.com/science/article/pii/S030439402200132X

Citation 11

https://www.sciencedirect.com/science/article/pii/S0168822719302050

Citation 12

https://www.sciencedirect.com/science/article/pii/S0168822719302177

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human IGF-1 concentrations in serum, plasma.

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