QuicKey Pro Human IgA (Immunoglobulin A) ELISA Kit

Product Specifications

Abbreviation

IgA

Cross Reactivity

This kit recognizes Human IgA in samples.No significant cross-reactivity or interference between Human IgA and analogues was observed

Target

Immunoglobulin A; IgA

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgA. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IgA are added to the micro ELISA plate wells. Human IgA in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IgA.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human IgA. The concentration of Human IgA in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, Plasma, Urine, Saliva

Detection Range

0.78-50 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.14 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.mdpi.com/1457378

Citation 02

https://www.mdpi.com/2304-8158/11/5/759

Citation 03

https://www.mdpi.com/2304-8158/10/7/1580

Citation 04

https://www.frontiersin.org/articles/10.3389/fphar.2020.588560/full?utm_source=researcher_app&utm_medium=referral&utm_campaign=RESR_MRKT_Researcher_inbound