QuicKey Pro Human T (Testosterone) ELISA Kit

Product Specifications

Abbreviation

Cross Reactivity

This kit recognizes Human T in samples.No significant cross-reactivity or interference between Human T and analogues was observed

Target

Testosterone; T

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal Transduction; Signal Transduction; Epigenetics and Nuclear Signaling; Developmental Biology

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human T are added to the micro ELISA plate wells. Human T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human T in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, Plasma, Urine, Saliva

Detection Range

0.63-40 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.24 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.frontiersin.org/articles/10.3389/fphys.2021.735986/full

Citation 02

https://www.sciencedirect.com/science/article/pii/S0378427421009024

Citation 03

https://rep.bioscientifica.com/view/journals/rep/162/1/REP-20-0643.xml

Citation 04

https://www.sciencedirect.com/science/article/pii/S0041008X1930016X

Citation 05

https://www.sciencedirect.com/science/article/pii/S0378427419302747

Citation 06

https://www.sciencedirect.com/science/article/pii/S1089860320301555

Citation 07

https://www.sciencedirect.com/science/article/pii/S1642431X2200105X

Citation 08

https://onlinelibrary.wiley.com/doi/abs/10.1111/and.14621

Citation 09

https://www.liebertpub.com/doi/abs/10.1089/ham.2020.0187

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human T concentrations in serum, plasma, saliva, urine.

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