QuicKey Pro Human Cortisol ELISA Kit

Product Specifications

Abbreviation

Cortisol

Cross Reactivity

This kit recognizes Human Cortisol in samples.No significant cross-reactivity or interference between Human Cortisol and analogues was observed

Target

11β;17α;21-Trihydroxypregn-4-ene-3;20-dione

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cell biology; Metabolism

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Cortisol are added to the micro ELISA plate wells. Human Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, Plasma, Urine, Saliva

Detection Range

6.25-400 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

2.87 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0269749122010363

Citation 02

https://www.sciencedirect.com/science/article/pii/S0956566316306765

Citation 03

https://www.sciencedirect.com/science/article/pii/S0889159121006267

Citation 04

https://link.springer.com/article/10.1186/s12970-020-00375-4

Citation 05

https://www.frontiersin.org/articles/10.3389/fphys.2021.735986/full

Citation 06

https://europepmc.org/articles/pmc9469133/bin/atm-10-16-894-rc.pdf?javascript_support=no

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384734/

Citation 08

https://www.hindawi.com/journals/ecam/2022/3874745/

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908583/

Citation 10

https://www.tandfonline.com/doi/abs/10.1080/01443615.2017.1400523

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human Cortisol concentrations in serum, plasma, saliva, urine.

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