QuicKey Pro Human CRP (C-Reactive Protein) ELISA Kit

Product Specifications

Abbreviation

CRP

UniProt

P02741

Cross Reactivity

This kit recognizes Human CRP in samples.No significant cross-reactivity or interference between Human CRP and analogues was observed

Target

C-Reactive Protein; CRP; PTX1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CRP are added to the micro ELISA plate wells. Human CRP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CRP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human CRP. The concentration of Human CRP in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

125-8000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

52.1 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.mdpi.com/2227-9059/9/12/1931

Citation 02

https://www.mdpi.com/2041788

Citation 03

https://pubmed.ncbi.nlm.nih.gov/32949690/

Citation 04

https://www.sciencedirect.com/science/article/pii/S0003267022007000

Citation 05

https://academic.oup.com/ndt/advance-article-abstract/doi/10.1093/ndt/gfaa335/6067632

Citation 06

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517782/

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcpe.13400

Citation 08

https://www.sciencedirect.com/science/article/pii/S0022395622001972

Citation 09

https://www.mdpi.com/2072-6643/12/9/2644

Citation 10

https://www.sciencedirect.com/science/article/pii/S0899900721001684

Citation 11

https://www.nature.com/articles/s41598-021-87583-3

Citation 12

https://www.karger.com/Article/Abstract/490246

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human CRP concentrations in serum, plasma.

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