Mini Sample Mouse MCP-2/CCL8 (Monocyte Chemotactic Protein 2) ELISA Kit
Product Specifications
Abbreviation
MCP-2
UniProt
Q9Z121
Cross Reactivity
This kit recognizes Rat SELE in samples. No significant cross-reactivity or interference between Rat SELE and analogues was observed.
Target
MCP-2; CCL8
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Immunology
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MCP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MCP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MCP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MCP-2. You can calculate the concentration of Mouse MCP-2 in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.25-2000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
10.45 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse MCP-2 concentrations in serum, plasma and other biological fluids.
Available Sizes
Frequently Asked Questions
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