Mini Sample Mouse IFN-γ (Interferon Gamma) ELISA Kit

Product Specifications

Abbreviation

IFN-γ

UniProt

P01580

Cross Reactivity

This kit recognizes Mouse IFN-γ in samples.No significant cross-reactivity or interference between Mouse IFN-γ and analogues was observed

Target

IFN-γ

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Immunology; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFN-γ. You can calculate the concentration of Mouse IFN-γ in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.53 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.202208923

Citation 02

https://www.sciencedirect.com/science/article/pii/S1385894722059460

Citation 03

https://www.sciencedirect.com/science/article/pii/S1385894722060995

Citation 04

https://www.science.org/doi/abs/10.1126/scitranslmed.aaz6667

Citation 05

https://www.sciencedirect.com/science/article/pii/S0142961222005427

Citation 06

https://www.science.org/doi/abs/10.1126/sciadv.abg8335

Citation 07

https://www.sciencedirect.com/science/article/pii/S2211383523000643

Citation 08

https://jitc.bmj.com/content/10/1/e003809.abstract

Citation 09

https://jitc.bmj.com/content/10/6/e004381.abstract

Citation 10

https://link.springer.com/article/10.1186/s12951-022-01287-y

Citation 11

https://pubs.acs.org/doi/abs/10.1021/acsami.2c14448

Citation 12

https://pubs.acs.org/doi/abs/10.1021/acsami.2c04894

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse IFN-γ concentrations in serum, plasma and other biological fluids.

Available Sizes

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