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Mini Sample Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit

Product Specifications

Abbreviation

VEGF-A

UniProt

Q00731

Cross Reactivity

This kit recognizes Mouse VEGF-A in samples.No significant cross-reactivity or interference between Mouse VEGF-A and analogues was observed

Target

VEGF

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Developmental biology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse VEGF-A. You can calculate the concentration of Mouse VEGF-A in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

3.21 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Product Datasheet

https://789.bio/ea/jvfz1s

Product MSDS

https://789.bio/eb/OWHuT0

Citation 01

https://www.nature.com/articles/s41551-020-00656-y

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13316

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1002/glia.24109

Citation 04

https://www.tandfonline.com/doi/abs/10.1080/10717544.2022.2096714

Citation 05

https://www.sciencedirect.com/science/article/pii/S0944711322005955

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1002/jbmr.3388

Citation 07

https://www.mdpi.com/1065770

Citation 08

https://www.tandfonline.com/doi/abs/10.1080/10717544.2022.2048134

Citation 09

https://www.sciencedirect.com/science/article/pii/S0753332218386372

Citation 10

https://www.frontiersin.org/articles/10.3389/fphar.2021.719823/full

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8370028/

Citation 12

https://link.springer.com/article/10.1186/s13023-022-02528-x

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse VEGF-A concentrations in serum, plasma and other biological fluids.

Available Sizes

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