Rat NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Product Specifications

Abbreviation

NT-proBNP

UniProt

P13205

Cross Reactivity

This kit recognizes Rat NT-proBNP in samples.No significant cross-reactivity or interference between Rat NT-proBNP and analogues was observed

Target

NT-Pro-BNP

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NT-proBNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NT-proBNP. You can calculate the concentration of Rat NT-proBNP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

37.5 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096912/

Citation 02

https://www.sciencedirect.com/science/article/pii/S0753332220313779

Citation 03

https://www.sciencedirect.com/science/article/pii/S0041008X21001861

Citation 04

https://www.frontiersin.org/articles/10.3389/fphar.2020.607384/full

Citation 05

https://www.sciencedirect.com/science/article/pii/S1319016421001183

Citation 06

https://www.mdpi.com/1422-0067/21/19/7286

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1111/vox.13191

Citation 08

https://www.hindawi.com/journals/ecam/2021/6635876/

Citation 09

https://www.hindawi.com/journals/jhe/2022/9494981/

Citation 10

https://www.sciencedirect.com/science/article/pii/S1570023219310931

Citation 11

https://www.tandfonline.com/doi/abs/10.1080/13880209.2020.1778043

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5324911/

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat NT-proBNP concentrations in serum, plasma and other biological fluids.

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