Rat MMP-9 (Matrix Metalloproteinase 9) ELISA Kit

Product Specifications

Abbreviation

MMP-9

UniProt

P50282

Cross Reactivity

This kit recognizes Rat MMP-9 in samples.No significant cross-reactivity or interference between Rat MMP-9 and analogues was observed

Target

MMP9; CLG4B; Gelatinase B; GELB; MANDP2;92kDa Type IV Collagenase;92 KDa Gelatinase

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-9. You can calculate the concentration of Rat MMP-9 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.69 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0753332222003377

Citation 02

https://www.sciencedirect.com/science/article/pii/S0753332220313779

Citation 03

https://www.sciencedirect.com/science/article/pii/S0753332222001111

Citation 04

https://www.hindawi.com/journals/omcl/2021/8836355/

Citation 05

https://www.mdpi.com/2076-3921/11/2/412

Citation 06

https://www.sciencedirect.com/science/article/pii/S0141813022007279

Citation 07

https://pubs.acs.org/doi/abs/10.1021/acs.biomac.0c01709

Citation 08

https://www.sciencedirect.com/science/article/pii/S0944711322006663

Citation 09

https://www.mdpi.com/995406

Citation 10

https://www.sciencedirect.com/science/article/pii/S105324981401448X

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973462/

Citation 12

https://europepmc.org/article/pmc/pmc8703111?javascript_support=no

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat MMP-9 concentrations in serum, plasma and other biological fluids.

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