Rat KIM-1 (Kidney Injury Molecule 1) ELISA Kit

Product Specifications

Abbreviation

KIM-1

UniProt

O54947

Cross Reactivity

This kit recognizes Rat KIM-1 in samples.No significant cross-reactivity or interference between Rat KIM-1 and analogues was observed

Target

HAVCR1; HAVCR; HAVCR-1; KIM-1; KIM1; TIM; TIM-1; TIM1; TIMD-1; TIMD1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Immunology; Microbiology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat KIM-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat KIM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat KIM-1. You can calculate the concentration of Rat KIM-1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/article/10.3390/ijms232315400

Citation 02

https://www.sciencedirect.com/science/article/pii/S0753332221003553

Citation 03

https://www.sciencedirect.com/science/article/pii/S0753332221007605

Citation 04

https://www.sciencedirect.com/science/article/pii/S1567576923000115

Citation 05

https://www.sciencedirect.com/science/article/pii/S1567576922010712

Citation 06

https://www.sciencedirect.com/science/article/pii/S094471132200099X

Citation 07

https://www.sciencedirect.com/science/article/pii/S0024320521011814

Citation 08

https://www.sciencedirect.com/science/article/pii/S0024320522003782

Citation 09

https://www.sciencedirect.com/science/article/pii/S0024320521005865

Citation 10

https://www.hindawi.com/journals/omcl/2020/2978340/

Citation 11

https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.201902943R

Citation 12

https://www.sciencedirect.com/science/article/pii/S0753332220306764

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat KIM-1 concentrations in serum, plasma and other biological fluids.

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