Rat COL1 (Collagen Type I) ELISA Kit

Product Specifications

Abbreviation

COL1

UniProt

P02454

Cross Reactivity

This kit recognizes Rat COL1 in samples.No significant cross-reactivity or interference between Rat COL1 and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat COL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat COL1. You can calculate the concentration of Rat COL1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.69 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://pubs.acs.org/doi/abs/10.1021/acs.jmedchem.2c00520

Citation 02

https://www.sciencedirect.com/science/article/pii/S0223523418300862

Citation 03

https://www.sciencedirect.com/science/article/pii/S0944711321003020

Citation 04

https://pubs.rsc.org/en/content/articlehtml/2019/tb/c8tb02456a

Citation 05

https://www.sciencedirect.com/science/article/pii/S0197018616300523

Citation 06

https://www.sciencedirect.com/science/article/pii/S0024320518304351

Citation 07

https://europepmc.org/article/med/32572894?javascript_support=no

Citation 08

https://onlinelibrary.wiley.com/doi/abs/10.1002/star.201700319

Citation 09

https://www.tandfonline.com/doi/abs/10.1080/10520295.2020.1758343

Citation 10

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8448625/

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat COL1 concentrations in serum, plasma and other biological fluids.

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