Rat GLP-1 (Glucagon Like Peptide 1) ELISA Kit

Product Specifications

Abbreviation

GLP-1

UniProt

P06883

Cross Reactivity

This kit recognizes Rat GLP-1 in samples.No significant cross-reactivity or interference between Rat GLP-1 and analogues was observed

Target

GLP1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cardiovascular; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GLP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GLP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GLP-1. You can calculate the concentration of Rat GLP-1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://link.springer.com/article/10.1007/s12035-017-0622-3

Citation 02

https://www.sciencedirect.com/science/article/pii/S0378874122009011

Citation 03

https://link.springer.com/content/pdf/10.1007/s00394-020-02249-6.pdf

Citation 04

https://www.mdpi.com/1422-0067/21/5/1746

Citation 05

https://www.sciencedirect.com/science/article/pii/S0944711320300994

Citation 06

https://www.sciencedirect.com/science/article/pii/S1319562X21005830

Citation 07

https://www.sciencedirect.com/science/article/pii/S2405844022021752

Citation 08

https://pubs.acs.org/doi/abs/10.1021/acs.jafc.9b02434

Citation 09

https://www.sciencedirect.com/science/article/pii/S0928098719304695

Citation 10

https://www.sciencedirect.com/science/article/pii/S221342201830218X

Citation 11

https://journals.sagepub.com/doi/abs/10.1177/15593258221114184

Citation 12

https://www.hindawi.com/journals/ecam/2021/5585952/

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat GLP-1 concentrations in serum, plasma and other biological fluids.

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