Rat PRL (Prolactin) ELISA Kit

Product Specifications

Abbreviation

PRL

UniProt

P01237

Cross Reactivity

This kit recognizes Rat PRL in samples.No significant cross-reactivity or interference between Rat PRL and analogues was observed

Target

Lactotrope; LTH; Luteotropic Hormone

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PRL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PRL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PRL. You can calculate the concentration of Rat PRL in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

3.13-200 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

1.88 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S2001037020304761

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8439717/

Citation 03

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8815076/

Citation 04

https://erc.bioscientifica.com/view/journals/erc/28/7/ERC-21-0137.xml

Citation 05

https://jme.bioscientifica.com/view/journals/jme/67/3/JME-21-0095.xml

Citation 06

https://www.sciencedirect.com/science/article/pii/S0300483X20301955

Citation 07

https://www.sciencedirect.com/science/article/pii/S037887412100221X

Citation 08

https://link.springer.com/article/10.1007/s11356-020-10324-y

Citation 09

https://www.sciencedirect.com/science/article/pii/S0018506X22000903

Citation 10

https://www.sciencedirect.com/science/article/pii/S0166432822003503

Citation 11

https://link.springer.com/article/10.1007/s12020-022-03010-1

Citation 12

https://onlinelibrary.wiley.com/doi/abs/10.1002/jbt.22741

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat PRL concentrations in serum, plasma and other biological fluids.

Available Sizes

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