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Rat C-P (C-peptide) ELISA Kit

Product Specifications

Abbreviation

C-P

UniProt

P01322, P01323

Cross Reactivity

This kit recognizes Rat C-P in samples.No significant cross-reactivity or interference between Rat C-P and analogues was observed

Target

Connecting Peptide

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Signaling transduction; Metabolism

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C-P. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C-P and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C-P, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat C-P. You can calculate the concentration of Rat C-P in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Product Datasheet

https://789.bio/ea/apwnrp

Product MSDS

https://789.bio/eb/bnDWrH

Citation 01

https://www.sciencedirect.com/science/article/pii/S0024320521012194

Citation 02

https://www.frontiersin.org/articles/10.3389/fcimb.2021.740236/full

Citation 03

https://www.sciencedirect.com/science/article/pii/S037887412100218X

Citation 04

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873975/

Citation 05

https://www.hindawi.com/journals/jdr/2022/1193392/

Citation 06

https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-022-03507-2

Citation 07

https://peerj.com/articles/11547/

Citation 08

https://link.springer.com/article/10.1007/s10967-023-08796-6

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat C-P concentrations in serum, plasma and other biological fluids.

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