Rat GH (Growth Hormone) ELISA Kit

Product Specifications

Abbreviation

UniProt

P01244

Cross Reactivity

This kit recognizes Rat GH in samples.No significant cross-reactivity or interference between Rat GH and analogues was observed

Target

GH1; GH-N; GHN; Somatotropin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Developmental biology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GH. You can calculate the concentration of Rat GH in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://diabetes.diabetesjournals.org/content/66/3/674.abstract

Citation 02

https://www.sciencedirect.com/science/article/pii/S0147651322010831

Citation 03

https://www.sciencedirect.com/science/article/pii/S0278691521002167

Citation 04

https://www.sciencedirect.com/science/article/pii/S2001037020304761

Citation 05

https://jme.bioscientifica.com/view/journals/jme/67/3/JME-21-0095.xml

Citation 06

https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/bee-venom-stimulates-hormone-secretion-in-rat-somatotroph-and-corticotroph-cells-digital-image-analysis-of-secretory-granules/212D9EFCBE0C09F4F8F14412E788C169