Rat ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit

Product Specifications

Abbreviation

ICAM-1; CD54

UniProt

Q00238

Cross Reactivity

This kit recognizes Rat ICAM-1/CD54 in samples.No significant cross-reactivity or interference between Rat ICAM-1/CD54 and analogues was observed

Target

BB2; P3.58

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ICAM-1/CD54. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ICAM-1/CD54, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ICAM-1/CD54. You can calculate the concentration of Rat ICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://pubs.acs.org/doi/abs/10.1021/acs.biomac.0c01709

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.29453

Citation 03

https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-021-01687-y

Citation 04

https://www.nature.com/articles/srep33390

Citation 05

https://journals.sagepub.com/doi/abs/10.1177/1535370220961038

Citation 06

https://www.tandfonline.com/doi/abs/10.1080/13880209.2022.2070221

Citation 07

https://www.tandfonline.com/doi/abs/10.1080/13880209.2021.1970199

Citation 08

https://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch190706

Citation 09

https://www.scielo.br/j/bja/a/85sFBX3697vhSJN54Bs7kqr/

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat ICAM-1/CD54 concentrations in serum, plasma and other biological fluids.

Available Sizes

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