Rat VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit

Product Specifications

Abbreviation

VEGF-A

UniProt

P16612

Cross Reactivity

This kit recognizes Rat VEGF-A in samples. No significant cross-reactivity or interference between Rat VEGF-A and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Developmental biology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VEGF-A. You can calculate the concentration of Rat VEGF-A in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S2452199X21001304

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201706769

Citation 03

https://link.springer.com/article/10.1007/s42765-022-00224-w

Citation 04

https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.202201661

Citation 05

https://www.sciencedirect.com/science/article/pii/S0142961220302313

Citation 06

https://www.sciencedirect.com/science/article/pii/S2590049823000097

Citation 07

https://www.sciencedirect.com/science/article/pii/S0264127522002623

Citation 08

https://www.sciencedirect.com/science/article/pii/S0944711322005955

Citation 09

https://www.sciencedirect.com/science/article/pii/S002432052200755X

Citation 10

https://stemcellres.biomedcentral.com/articles/10.1186/s13287-020-01733-4

Citation 11

https://pubs.acs.org/doi/abs/10.1021/acs.biomac.0c01709

Citation 12

https://www.hindawi.com/journals/omcl/2022/7319563/

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat VEGF-A concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

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