Rat INS (Insulin) ELISA Kit

Product Specifications

Abbreviation

INS

UniProt

P01322, P01323

Cross Reactivity

This kit recognizes Rat INS in samples. No significant cross-reactivity or interference between Rat INS and analogues was observed

Target

IDDM2; ILPR; IRDN; MODY10

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat INS. You can calculate the concentration of Rat INS in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Cell culture supernatant and tissue homogenate

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.frontiersin.org/articles/10.3389/fcell.2021.646113/full

Citation 02

https://www.sciencedirect.com/science/article/pii/S0963996921001861

Citation 03

https://www.mdpi.com/2005176

Citation 04

https://www.mdpi.com/1422-0067/21/19/7161

Citation 05

https://www.mdpi.com/2218-273X/8/4/149

Citation 06

https://journals.physiology.org/doi/abs/10.1152/ajpendo.00093.2021

Citation 07

https://www.sciencedirect.com/science/article/pii/S0361923021000708

Citation 08

https://www.sciencedirect.com/science/article/pii/S0303720721003063

Citation 09

https://www.sciencedirect.com/science/article/pii/S0014482722003998

Citation 10

https://www.sciencedirect.com/science/article/pii/S1875536414600431

Citation 11

https://www.karger.com/Article/Abstract/480395

Citation 12

https://link.springer.com/article/10.1007/s00424-020-02443-9

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat INS concentrations in cell culture supernatant and tissue homogenate.

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