Rat ACE (Angiotensin I Converting Enzyme) ELISA Kit

Product Specifications

Abbreviation

ACE

UniProt

P47820

Cross Reactivity

This kit recognizes Rat ACE in samples. No significant cross-reactivity or interference between Rat ACE and analogues was observed

Target

ACE1; CD143; DCP; DCP1; ICH; MVCD3

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular; Metabolism; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ACE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ACE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ACE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ACE. You can calculate the concentration of Rat ACE in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0753332221011070

Citation 02

https://europepmc.org/article/pmc/pmc8678489?javascript_support=no

Citation 03

https://www.hindawi.com/journals/omcl/2018/5158243/abs/

Citation 04

https://www.sciencedirect.com/science/article/pii/S0165037823001298

Citation 05

https://www.sciencedirect.com/science/article/pii/S0024320520314661

Citation 06

https://www.tandfonline.com/doi/abs/10.1080/13880209.2020.1778043

Citation 07

https://www.mdpi.com/1420-3049/22/11/1956

Citation 08

https://link.springer.com/article/10.1007/s11033-019-04730-w

Citation 09

https://www.tandfonline.com/doi/abs/10.1080/13813455.2020.1780263

Citation 10

https://www.tandfonline.com/doi/abs/10.1080/07420528.2019.1610419

Citation 11

https://www.tandfonline.com/doi/abs/10.1080/10641963.2018.1506467

Citation 12

https://physoc.onlinelibrary.wiley.com/doi/abs/10.14814/phy2.15598

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat ACE concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

CQA9893-01 30 µL

Anti-CCZ1 Antibody

Ask

View Details

CQA9893-02 50 μL

Anti-CCZ1 Antibody

Ask

View Details

CQA9893-03 100 µL

Anti-CCZ1 Antibody

Ask

View Details

CQA9893-04 200 µL

Anti-CCZ1 Antibody

Ask

View Details

SL0129Hu-01 48 Tests

Human Ankyrin B (Ank-B) ELISA Kit

Ask

View Details

SL0129Hu-02 96 Tests

Human Ankyrin B (Ank-B) ELISA Kit

Ask

View Details