Rat CTXI (Cross Linked C-telopeptide of Type I Collagen) ELISA Kit

Product Specifications

Abbreviation

CTXI

UniProt

P02454

Cross Reactivity

This kit recognizes Rat CTXI in samples. No significant cross-reactivity or interference between Rat CTXI and analogues was observed

Target

CTX-I; CTX1; ICTP

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Metabolism; Cardiovascular; Cancer

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CTXI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTXI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTXI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CTXI. You can calculate the concentration of Rat CTXI in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-021-00976-4

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8491742/

Citation 03

https://link.springer.com/article/10.1007/s10565-022-09713-5

Citation 04

https://europepmc.org/article/pmc/pmc9434695?javascript_support=no

Citation 05

https://www.sciencedirect.com/science/article/pii/S0006295222003781

Citation 06

https://europepmc.org/article/pmc/pmc9513524?javascript_support=no

Citation 07

https://www.mdpi.com/2072-6643/14/9/1683

Citation 08

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8579055/

Citation 09

https://www.frontiersin.org/articles/10.3389/fphar.2017.00558/full

Citation 10

https://www.sciencedirect.com/science/article/pii/S8756328220303422

Citation 11

https://www.sciencedirect.com/science/article/pii/S8756328220303835

Citation 12

https://www.mdpi.com/648596

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat CTXI concentrations in serum, plasma and other biological fluids.

Available Sizes

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