Rat Ang-II (Angiotensin II) ELISA Kit

Product Specifications

Abbreviation

Ang-II

UniProt

P01015 (Angiotensinogen,25 – 32)

Cross Reactivity

This kit recognizes Rat Ang-II in samples.No significant cross-reactivity or interference between Rat Ang-II and analogues was observed

Target

Ang-II; Angiotensin-2

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Ang-II. During the reaction, Rat Ang-II in the sample or standard competes with a fixed amount of Rat Ang-II on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat Ang-II. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Ang-II in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.hindawi.com/journals/omcl/2022/6371048/

Citation 02

https://www.sciencedirect.com/science/article/pii/S0009279721003707

Citation 03

https://www.mdpi.com/2038840

Citation 04

https://www.sciencedirect.com/science/article/pii/S0024320521005269

Citation 05

https://europepmc.org/article/med/35991671?javascript_support=no

Citation 06

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024534/

Citation 07

https://journals.lww.com/jhypertension/FullText/2017/07000/Adjunctive_therapy_with_statins_reduces_residual.17.aspx

Citation 08

https://www.sciencedirect.com/science/article/pii/S0269749117348984

Citation 09

https://www.frontiersin.org/articles/10.3389/fphar.2020.00094/full

Citation 10

https://journals.sagepub.com/doi/abs/10.1177/1535370219854325

Citation 11

https://www.sciencedirect.com/science/article/pii/S175646461930204X

Citation 12

https://link.springer.com/article/10.1007/s12011-021-02804-0

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat Ang-II concentrations in serum, plasma and other biological fluids.

Available Sizes

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