Rat PINP (Procollagen I N-Terminal Propeptide) ELISA Kit

Product Specifications

Abbreviation

PINP

UniProt

P02454 (COL1A1, 23-151)

Cross Reactivity

This kit recognizes Rat PINP in samples. No significant cross-reactivity or interference between Rat PINP and analogues was observed

Target

P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Signal Transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PINP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PINP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PINP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PINP. You can calculate the concentration of Rat PINP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

37.5 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-021-00976-4

Citation 02

https://europepmc.org/article/pmc/pmc9434695?javascript_support=no

Citation 03

https://www.sciencedirect.com/science/article/pii/S0006295222003781

Citation 04

https://www.nature.com/articles/cddis2016249

Citation 05

https://www.karger.com/Article/Abstract/489653

Citation 06

https://www.sciencedirect.com/science/article/pii/S0939641121001892

Citation 07

https://www.nature.com/articles/srep33803/

Citation 08

https://www.sciencedirect.com/science/article/pii/S0753332220306417

Citation 09

https://www.sciencedirect.com/science/article/pii/S8756328220303835

Citation 10

https://www.sciencedirect.com/science/article/pii/S8756328220303422

Citation 11

https://www.sciencedirect.com/science/article/pii/S0378874120335327

Citation 12

https://link.springer.com/article/10.1007/s10787-017-0432-2

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat PINP concentrations in serum, plasma and other biological fluids.

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