Rat Aβ1-42 (Amyloid Beta 1-42) ELISA Kit

Product Specifications

Abbreviation

Aβ1-42

UniProt

P08592 (APP,672-713)

Cross Reactivity

This kit recognizes Rat Aβ1-42 in samples. No significant cross-reactivity or interference between Rat Aβ1-42 and analogues was observed

Target

Aβ42; Amyloid Beta 42

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Aβ1-42. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Aβ1-42 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Aβ1-42, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Aβ1-42. You can calculate the concentration of Rat Aβ1-42 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0889159115300192

Citation 02

https://link.springer.com/content/pdf/10.1007/s13311-020-00841-6.pdf

Citation 03

https://www.hindawi.com/journals/omcl/2022/3344569/

Citation 04

https://europepmc.org/article/med/35222337?javascript_support=no

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286848/

Citation 06

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5707060/

Citation 07

https://www.frontiersin.org/articles/10.3389/fnagi.2017.00347/full

Citation 08

https://www.nature.com/articles/s41598-019-46558-1

Citation 09

https://onlinelibrary.wiley.com/doi/abs/10.1111/jre.12929

Citation 10

https://link.springer.com/article/10.1007/s12264-018-00336-7

Citation 11

https://www.sciencedirect.com/science/article/pii/S0378874121008278

Citation 12

https://link.springer.com/article/10.1007/s11356-020-11101-7

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat Aβ1-42 concentrations in serum, plasma and other biological fluids.

Available Sizes

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