Rat CKMB (Creatine Kinase MB Isoenzyme) ELISA Kit

Product Specifications

Abbreviation

CKMB

UniProt

P00564 (CKM) &P07335 (CKB)

Cross Reactivity

This kit recognizes Rat CKMB in samples. No significant cross-reactivity or interference between Rat CKMB and analogues was observed

Target

CKMB

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CKMB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CKMB. You can calculate the concentration of Rat CKMB in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41467-020-20844-3

Citation 02

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01639-8

Citation 03

https://www.hindawi.com/journals/omcl/2022/1090893/

Citation 04

https://www.sciencedirect.com/science/article/pii/S0753332222008265

Citation 05

https://www.sciencedirect.com/science/article/pii/S0024320522008232

Citation 06

https://www.ahajournals.org/doi/abs/10.1161/JAHA.121.023378

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099415/

Citation 08

https://portlandpress.com/clinsci/article-abstract/130/5/377/77812

Citation 09

https://www.sciencedirect.com/science/article/pii/S0014299922004332

Citation 10

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.16491

Citation 11

https://cardiab.biomedcentral.com/articles/10.1186/s12933-016-0460-z

Citation 12

https://link.springer.com/article/10.1007/s11356-021-15409-w

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat CKMB concentrations in serum, plasma and other biological fluids.

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