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Rat TNNI3/cTn-I (Troponin I Type 3, Cardiac) ELISA Kit

Product Specifications

Abbreviation

TNNI3; cTn-I

UniProt

P23693

Cross Reactivity

This kit recognizes Rat TNNI3/cTn-I in samples. No significant cross-reactivity or interference between Rat TNNI3/cTn-I and analogues was observed

Target

TNNC1; cTnI; CMD1FF; CMD2A; CMH7; RCM1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Developmental biology; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TNNI3/cTn-I. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNNI3/cTn-I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNNI3/cTn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TNNI3/cTn-I. You can calculate the concentration of Rat TNNI3/cTn-I in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0142961221003082

Citation 02

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01639-8

Citation 03

https://www.sciencedirect.com/science/article/pii/S0753332222010629

Citation 04

https://www.hindawi.com/journals/omcl/2022/1090893/

Citation 05

https://www.sciencedirect.com/science/article/pii/S0024320522008232

Citation 06

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099415/

Citation 07

https://www.tandfonline.com/doi/abs/10.1080/13510002.2022.2158526

Citation 08

https://www.sciencedirect.com/science/article/pii/S094471132100341X

Citation 09

https://www.ahajournals.org/doi/abs/10.1161/JAHA.120.019837

Citation 10

https://www.nature.com/articles/s41401-019-0301-1

Citation 11

https://link.springer.com/article/10.1007/s10753-020-01404-9

Citation 12

https://www.sciencedirect.com/science/article/pii/S0014299922001170

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat TNNI3/cTn-I concentrations in serum, plasma and other biological fluids.

Available Sizes

Frequently Asked Questions

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