Rat BDNF (Brain Derived Neurotrophic Factor) ELISA Kit

Product Specifications

Abbreviation

BDNF

UniProt

P23363

Cross Reactivity

This kit recognizes Rat BDNF in samples. No significant cross-reactivity or interference between Rat BDNF and analogues was observed

Target

ANON2; BULN2

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BDNF. You can calculate the concentration of Rat BDNF in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201706769

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.202202785

Citation 03

https://www.sciencedirect.com/science/article/pii/S2590049823000097

Citation 04

https://www.sciencedirect.com/science/article/pii/S0753332223000264

Citation 05

https://www.mdpi.com/2076-3921/11/11/2154

Citation 06

https://link.springer.com/article/10.1007/s13311-021-01140-4

Citation 07

https://www.mdpi.com/article/10.3390/ijms24010766

Citation 08

https://www.frontiersin.org/articles/10.3389/fcimb.2022.945851/full?utm_source=dlvr.it&utm_medium=twitter

Citation 09

https://www.mdpi.com/1422-0067/22/10/5400

Citation 10

https://pubs.rsc.org/en/content/articlelanding/2021/fo/d1fo01720a

Citation 11

https://link.springer.com/article/10.1007/s12035-022-02977-2

Citation 12

https://pubs.acs.org/doi/abs/10.1021/acschemneuro.2c00270

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat BDNF concentrations in serum, plasma and other biological fluids.

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