Rat VIP (Vasoactive Intestinal Peptide) ELISA Kit

Product Specifications

Abbreviation

VIP

UniProt

P01283 (125-152)

Cross Reactivity

This kit recognizes Rat VIP in samples.No significant cross-reactivity or interference between Rat VIP and analogues was observed

Target

PHM27

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Neuroscience

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat VIP. During the reaction, Rat VIP in the sample or standard competes with a fixed amount of Rat VIP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat VIP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat VIP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.69 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0944711321004153

Citation 02

https://cmjournal.biomedcentral.com/articles/10.1186/s13020-021-00562-8

Citation 03

https://www.sciencedirect.com/science/article/pii/S0378874120332967

Citation 04

https://www.sciencedirect.com/science/article/pii/S0378874121001513

Citation 05

https://www.sciencedirect.com/science/article/pii/S0006899319304202

Citation 06

https://www.scielo.br/scielo.php?pid=S0104-42302019000901188&script=sci_arttext

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat VIP concentrations in Serum, plasma and other biological fluids.

Available Sizes

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