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Rat NFE2L2 (Nuclear Factor, Erythroid Derived 2, Like 2) ELISA Kit

Product Specifications

Abbreviation

NFE2L2

UniProt

O54968

Cross Reactivity

This kit recognizes Rat NFE2L2 in samples. No significant cross-reactivity or interference between Rat NFE2L2 and analogues was observed

Target

Nrf2

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NFE2L2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NFE2L2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NFE2L2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NFE2L2. You can calculate the concentration of Rat NFE2L2 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0753332222001664

Citation 02

https://www.sciencedirect.com/science/article/pii/S0014299921001977

Citation 03

https://www.sciencedirect.com/science/article/pii/S0899900722003264

Citation 04

https://link.springer.com/article/10.1007/s10787-020-00785-5

Citation 05

https://link.springer.com/article/10.1007/s11010-022-04639-2

Citation 06

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0272124

Citation 07

https://www.sciencedirect.com/science/article/pii/S0306452223000052

Citation 08

https://link.springer.com/article/10.1007/s10753-019-01028-8

Citation 09

https://www.sciencedirect.com/science/article/pii/S2214750021001207

Citation 10

https://www.sciencedirect.com/science/article/pii/S2666027X22000354

Citation 11

https://link.springer.com/article/10.1007/s40200-021-00924-x

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat NFE2L2 concentrations in serum, plasma and other biological fluids.

Available Sizes

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