Rat TRACP-5b (Tartrate Resistant Acid Phosphatase 5b) ELISA Kit

Product Specifications

Abbreviation

TRACP-5b

UniProt

P29288

Cross Reactivity

This kit recognizes Rat TRACP-5b in samples. No significant cross-reactivity or interference between Rat TRACP-5b and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Immunology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TRACP-5b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TRACP-5b. You can calculate the concentration of Rat TRACP-5b in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.63-40 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.38 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-021-00976-4

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8579055/

Citation 03

https://www.sciencedirect.com/science/article/pii/S0024320522001060

Citation 04

https://www.mdpi.com/648596

Citation 05

https://www.sciencedirect.com/science/article/pii/S0753332220304960

Citation 06

https://www.sciencedirect.com/science/article/pii/S0960076015300157

Citation 07

https://link.springer.com/article/10.1007/s12020-022-03199-1

Citation 08

https://josr-online.biomedcentral.com/articles/10.1186/s13018-022-03162-w

Citation 09

https://journals.sagepub.com/doi/abs/10.1136/acupmed-2016-011268

Citation 10

https://portlandpress.com/bioscirep/article-abstract/35/4/e00239/56188

Citation 11

https://www.spandidos-publications.com/10.3892/etm.2015.2553

Citation 12

https://link.springer.com/article/10.1007/s13596-019-00377-x

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat TRACP-5b concentrations in serum, plasma and other biological fluids.

Available Sizes

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