Rat GHRL (Ghrelin) ELISA Kit

Product Specifications

Abbreviation

GHRL

UniProt

Q9QYH7 aa:24-51

Cross Reactivity

This kit recognizes Rat GHRL in samples.No significant cross-reactivity or interference between Rat GHRL and analogues was observed

Target

Obestatin Preprohormone; MTLRP; Ghrelin

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat GHRL. During the reaction, Rat GHRL in the sample or standard competes with a fixed amount of Rat GHRL on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat GHRL. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat GHRL in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0144861722002089

Citation 02

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo01744f

Citation 03

https://www.nature.com/articles/s41598-021-98605-5

Citation 04

https://www.sciencedirect.com/science/article/pii/S0306453022002037

Citation 05

https://www.nature.com/articles/s41598-020-77672-0

Citation 06

https://www.mdpi.com/1420-3049/23/4/794

Citation 07

https://www.tandfonline.com/doi/abs/10.1080/13813455.2019.1680697

Citation 08

https://www.sciencedirect.com/science/article/pii/S0304394017301921

Citation 09

https://www.sciencedirect.com/science/article/pii/S221342201830218X

Citation 10

https://www.tandfonline.com/doi/abs/10.1080/10520295.2021.1896780

Citation 11

https://www.degruyter.com/document/doi/10.1515/biol-2022-0070/html

Citation 12

https://link.springer.com/article/10.1007/s11756-022-01266-6

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat GHRL concentrations in serum, plasma and other biological fluids.

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