Rat PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) ELISA Kit

Product Specifications

Abbreviation

PTGS2; COX-2

UniProt

P35355

Cross Reactivity

This kit recognizes Rat PTGS2/COX-2 in samples. No significant cross-reactivity or interference between Rat PTGS2/COX-2 and analogues was observed

Target

COX2; GRIPGHS; PGG/HS; PGHS-2; PHS-2; hCox-2

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PTGS2/COX-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PTGS2/COX-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PTGS2/COX-2. You can calculate the concentration of Rat PTGS2/COX-2 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

125-8000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.hindawi.com/journals/omcl/2021/9966663/

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005812/

Citation 03

https://www.sciencedirect.com/science/article/pii/S0141813022007279

Citation 04

https://www.frontiersin.org/articles/10.3389/fendo.2021.652807/full

Citation 05

https://link.springer.com/article/10.1007/s11356-022-23324-x

Citation 06

https://www.sciencedirect.com/science/article/pii/S0141813020338824

Citation 07

https://link.springer.com/article/10.1007/s10787-020-00785-5

Citation 08

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695383/

Citation 09

https://www.mdpi.com/1420-3049/27/7/2224

Citation 10

https://www.sciencedirect.com/science/article/pii/S0014299921001977

Citation 11

https://www.sciencedirect.com/science/article/pii/S0161813X22001292

Citation 12

https://www.tandfonline.com/doi/abs/10.1080/13510002.2022.2074128

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat PTGS2/COX-2 concentrations in serum, plasma and other biological fluids.

Available Sizes

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